A research and diagnostic laboratory specialising in Vitamin D biology research in companion, production and wild animals

Vitamin D metabolite assay service

The VitDAL have developed an ultra-high performance liquid chromatography – mass spectrometry (UHPLC-MS) assay to accurately key vitamin D metabolites in serum and plasma. These assays are critically important to the VitDAL research programme and are also available as an external service to collaborators and clinicians. We have established a methodology which allows us to accurately quantify the major vitamin D metabolite, 25-hydroxyvitamin D (25(OH)D), which is widely used to assess vitamin D status. Currently, we are optimising a UHPLC-MS system  to allow us to accurately measure several other biologically relevant vitamin D metabolites, notably C-3 epimers of 25-hydroxyvitamin D, which have been previously shown to interfere with 25(OH)D measurements. This service aims to provide a robust, multi-species approach to assess vitamin D status that will not only support our research into vitamin D biology but will also provide clinically relevant data which will assist in patient management.

VitDAL submission form (Word doc, 127KB)

VitDAL submission form (PDF, 153KB)

If you would like to use the VitDAL assay service please contact Professor Richard Mellanby at Richard.Mellanby@ed.ac.uk or use the contact page on the VitDAL website. Please submitted samples from fasted patients to avoid lipaemia related assay interference.



latest news

VitDAL publishes new paper

VitDAL has published its latest research on the immune-modulating properties of vitamin D. We have explored the effects of the active vitamin D metabolite...

VitDAL publishes new paper

VitDAL have published their latest paper today on vitamin D status in dogs with babaesia in collaboration with colleagues at the University of Pretoria,...

VitDAL publishes new paper

The VitDAL published their latest study on vitamin D and health outcomes this week. The work was a collaborative study between R(D)SVS, Roslin Institute and SRUC and explored the relationship...