Vitamin D metabolite assay service

The VitDAL have developed an ultra-high performance liquid chromatography – mass spectrometry (UHPLC-MS) assay to accurately key vitamin D metabolites in serum and plasma. These assays are critically important to the VitDAL research programme and are also available as an external service to collaborators and clinicians. We have established a methodology which allows us to accurately quantify the major vitamin D metabolite, 25-hydroxyvitamin D (25(OH)D), which is widely used to assess vitamin D status. Currently, we are optimising a UHPLC-MS system  to allow us to accurately measure several other biologically relevant vitamin D metabolites, notably C-3 epimers of 25-hydroxyvitamin D, which have been previously shown to interfere with 25(OH)D measurements. This service aims to provide a robust, multi-species approach to assess vitamin D status that will not only support our research into vitamin D biology but will also provide clinically relevant data which will assist in patient management.

VitDAL submission form (Word doc, 127KB)

VitDAL submission form (PDF, 153KB)

If you would like to use the VitDAL assay service please contact Professor Richard Mellanby at or use the contact page on the VitDAL website. Please submitted samples from fasted patients to avoid lipaemia related assay interference.